Differential proteomic response of Agaricus bisporus and Trichoderma aggressivum f. europaeum to Bacillus velezensis supernatant

Trichoderma aggressivum, a mycopathogen causing green mould disease, is major problem in Agaricus bisporus cultivation due to crop loss, and resistance chemical fungicides. There an urgent need for novel biological ways control mycopathogens without affecting the growth of A. bisporus. Bacteria from mushroom-casing environment were identified tested antagonistic effect on T. aggressivum. Bacillus velezensis produced large zone inhibition its supernatant inhibited aggressivum [?37%], slightly stimulated [+2%]. Label free quantitative-proteomic (LFQ) analysis changes abundance proteins following exposure B. indicated increased associated with catabolic processing amino acids (40-fold), oxidase (14-fold), oxidoreductase (13-fold, 4-fold) hydrolases (3-fold). Proteins that decreased relative antioxidants (29-fold), NTF2 domain containing protein (17-fold), 60S ribosomal L-13 glucoamylase (13-fold), proteasome subunit (11-fold) other (9-fold). LFQ revealed exposing led decrease in: prohibitin 6-fold), proteasomal (11-fold), cytosolic adaptor (5-fold), aldehyde dehydrogenase (4-fold), DLH domain-containing (4-fold) PKS_ER The results indicate was not under stress upon contact velezensis. Whereas detrimental shown by damage-preventing stress.